Visualizing specific epigenetic marks at single gene loci is now possible in individual cells.
THE TECHNIQUE: A gene of interest is hybridized with a biotin-tagged DNA probe (red). Next, an anti-biotin antibody (pink) and an antibody (blue) recognizing an epigenetic mark—e.g., histone H3 methylation—are applied. These antibodies are then each tagged with PLA antibodies (orange and yellow). If the biotin and epigenetic mark are in close proximity, the two PLA antibodies will interact and create a signal detectable with a fluorescent DNA probe.
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