Precision Epigenetics: a new front

See on Scoop.itMedicine in Pictures

Visualizing specific epigenetic marks at single gene loci is now possible in individual cells.


THE TECHNIQUE: A gene of interest is hybridized with a biotin-tagged DNA probe (red). Next, an anti-biotin antibody (pink) and an antibody (blue) recognizing an epigenetic mark—e.g., histone H3 methylation—are applied. These antibodies are then each tagged with PLA antibodies (orange and yellow). If the biotin and epigenetic mark are in close proximity, the two PLA antibodies will interact and create a signal detectable with a fluorescent DNA probe.

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Indian Health Journal is a online initiative by Indian Healthcare IT company Plus91 Technologies to promote information sharing across healthcare domains through original articles submitted by various authors. These will be under various categories ranging from Medicine, Pharmaceuticals, IT, Medical Equipment and Health Infrastructure. Opinions from the brightest, Views from those in the hot seat, or just pure innovators will be adding articles here.
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